caption a7 antibiotic susceptibility  (ATCC)

Journal: Applied and Environmental Microbiology

Article Title: High-Level Antibiotic Tolerance of a Clinically Isolated Enterococcus faecalis Strain

doi: 10.1128/AEM.02083-20

Figure Lengend Snippet: Antibiotic susceptibility of UM001B, ATCC 29212, and B. subtilis 168. Van (A), Tet (B), and Amp (C) were tested at different concentrations. Exponential-phase planktonic cells cultured in LB medium were harvested and treated with each antibiotic for 3.5 h at 37°C, with shaking at 200 rpm. Each condition was tested with at least three biological replicates (n ≥ 3).

Article Snippet: Such high-level multidrug tolerance is remarkable and deserves further study. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 Antibiotic susceptibility of UM001B, ATCC 29212, and B. subtilis 168.

Techniques: Cell Culture

Journal: Journal of Bacteriology

Article Title: Antibiotic Susceptibility of Escherichia coli Cells during Early-Stage Biofilm Formation

doi: 10.1128/JB.00034-19

Figure Lengend Snippet: E. coli antibiotic susceptibility during early stages of biofilm formation. (A and B) Reduction of surface-attached E. coli RP437 cells by 1-h treatment with 200-μg/ml Amp (A) or 10-μg/ml Ofx (B). (C) Reduction of surface-attached uropathogenic E. coli ATCC 53505 cells by 1-h treatment with 200-μg/ml Amp. (D) Number of surface-attached E. coli RP437 cells on glass surfaces. Dotted lines indicate the time points when major changes in antibiotic susceptibility occurred, which correspond to the three phases (I, II, and III) marked in panels A, B, and C. E. coli biofilms were formed on glass surfaces. The antibiotic susceptibility was tested in 0.85% NaCl solution (no carbon source) to minimize the effects of cell growth. At least five biological replicates were tested for each data point.

Article Snippet: However, before entering this stage, the antibiotic susceptibility of attached E. coli RP437 cells exhibited a dynamic change over time ( and and Fig. S2), during which antibiotic susceptibility first increased and reached a peak level before decreasing as the biofilm matured. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 E. coli antibiotic susceptibility during early stages of biofilm formation. (A and B) Reduction of surface-attached E. coli RP437 cells by 1-h treatment with 200-μg/ml Amp (A) or 10-μg/ml Ofx (B). (C) Reduction of surface-attached uropathogenic E. coli ATCC 53505 cells by 1-h treatment with 200-μg/ml Amp. (D) Number of surface-attached E. coli RP437 cells on glass surfaces.

Techniques:

Journal: Journal of Bacteriology

Article Title: Antibiotic Susceptibility of Escherichia coli Cells during Early-Stage Biofilm Formation

doi: 10.1128/JB.00034-19

Figure Lengend Snippet: The concentration of Ofx in surface-attached and planktonic cells at 15 min after inoculation (***, P < 0.0005). The antibiotic treatment was conducted in 0.85% NaCl solution. E. coli RP437 biofilms were formed on glass surfaces in LB medium, and each condition was tested with at least three biological replicates.

Article Snippet: However, before entering this stage, the antibiotic susceptibility of attached E. coli RP437 cells exhibited a dynamic change over time ( and and Fig. S2), during which antibiotic susceptibility first increased and reached a peak level before decreasing as the biofilm matured. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 E. coli antibiotic susceptibility during early stages of biofilm formation. (A and B) Reduction of surface-attached E. coli RP437 cells by 1-h treatment with 200-μg/ml Amp (A) or 10-μg/ml Ofx (B). (C) Reduction of surface-attached uropathogenic E. coli ATCC 53505 cells by 1-h treatment with 200-μg/ml Amp. (D) Number of surface-attached E. coli RP437 cells on glass surfaces.

Techniques: Concentration Assay

Journal: Journal of Bacteriology

Article Title: Antibiotic Susceptibility of Escherichia coli Cells during Early-Stage Biofilm Formation

doi: 10.1128/JB.00034-19

Figure Lengend Snippet: Cell-cell interactions affected the Amp susceptibility of attached cells. (A) Representative fluorescence images of 2-h patterned biofilms treated with 200-μg/ml Amp and labeled with live/dead staining (bar, 10 μm). The antibiotic treatment was conducted in 0.85% NaCl solution. (B) Percentage of 2-h patterned biofilm cells (W = 20 μm and D = 2, 5, or 10 μm) killed by 200-μg/ml Amp (*, P < 0.05; **, P < 0.005; and ***, P < 0.0005). E. coli RP437 biofilms were formed on gold-coated glass surfaces in LB medium, and each condition was tested with three biological replicates.

Article Snippet: However, before entering this stage, the antibiotic susceptibility of attached E. coli RP437 cells exhibited a dynamic change over time ( and and Fig. S2), during which antibiotic susceptibility first increased and reached a peak level before decreasing as the biofilm matured. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 E. coli antibiotic susceptibility during early stages of biofilm formation. (A and B) Reduction of surface-attached E. coli RP437 cells by 1-h treatment with 200-μg/ml Amp (A) or 10-μg/ml Ofx (B). (C) Reduction of surface-attached uropathogenic E. coli ATCC 53505 cells by 1-h treatment with 200-μg/ml Amp. (D) Number of surface-attached E. coli RP437 cells on glass surfaces.

Techniques: Fluorescence, Labeling, Staining

Journal: Journal of Bacteriology

Article Title: Antibiotic Susceptibility of Escherichia coli Cells during Early-Stage Biofilm Formation

doi: 10.1128/JB.00034-19

Figure Lengend Snippet: Cells embedded in early-stage biofilms remained active and sensitive to antibiotics if dispersed prior to antibiotic treatment. (A) Representative fluorescence images of E. coli RP437, E. coli AR3110 (wild type), E. coli AR182 (ΔbscA), and E. coli AR282 (ΔcsgB) biofilms at different time points (1, 3, 16, and 24 h) during biofilm formation. The embedded cells were labeled with SYTO9, and the cellulose in the biofilm matrix was labeled with Congo red. (B and C) Susceptibility of different cell populations (detached biofilm cells and planktonic cells in the same static biofilm cultures) to Amp (B) or Ofx (C) during early events of biofilm formation. The susceptibility of planktonic cells was repeated (from Fig. 1A and ​andB)B) in these two panels as references for comparison. The antibiotic treatment was conducted in 0.85% NaCl solutions. (D) Representative fluorescence images of E. coli RP437 biofilms. Alexa Fluor 594-WGA stains sialic acid and N-acetyl-d-glucosamine in biofilm matrix, while SYTO9 stains surface-attached E. coli RP437 cells. E. coli RP437 biofilms were formed on glass surfaces in LB medium, and each condition was tested with three biological replicates (n = 3).

Article Snippet: However, before entering this stage, the antibiotic susceptibility of attached E. coli RP437 cells exhibited a dynamic change over time ( and and Fig. S2), during which antibiotic susceptibility first increased and reached a peak level before decreasing as the biofilm matured. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 E. coli antibiotic susceptibility during early stages of biofilm formation. (A and B) Reduction of surface-attached E. coli RP437 cells by 1-h treatment with 200-μg/ml Amp (A) or 10-μg/ml Ofx (B). (C) Reduction of surface-attached uropathogenic E. coli ATCC 53505 cells by 1-h treatment with 200-μg/ml Amp. (D) Number of surface-attached E. coli RP437 cells on glass surfaces.

Techniques: Fluorescence, Labeling, Comparison

Journal: Journal of Bacteriology

Article Title: Antibiotic Susceptibility of Escherichia coli Cells during Early-Stage Biofilm Formation

doi: 10.1128/JB.00034-19

Figure Lengend Snippet: A hypothetic model of antibiotic susceptibility during early-stage biofilm formation. Phases I, II, and III in this diagram are the main findings from this study. Cells with dotted lines in the cell membrane represent these initially attached cells or cells in interactions with increased membrane permeability. The mechanisms in biofilm maturation and dispersion are based on literature. They are included here to show the whole process of biofilm formation and associated antibiotic tolerance.

Article Snippet: However, before entering this stage, the antibiotic susceptibility of attached E. coli RP437 cells exhibited a dynamic change over time ( and and Fig. S2), during which antibiotic susceptibility first increased and reached a peak level before decreasing as the biofilm matured. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 E. coli antibiotic susceptibility during early stages of biofilm formation. (A and B) Reduction of surface-attached E. coli RP437 cells by 1-h treatment with 200-μg/ml Amp (A) or 10-μg/ml Ofx (B). (C) Reduction of surface-attached uropathogenic E. coli ATCC 53505 cells by 1-h treatment with 200-μg/ml Amp. (D) Number of surface-attached E. coli RP437 cells on glass surfaces.

Techniques: Membrane, Permeability, Dispersion